Carbapenems Resistance Klebsiella Species Isolated from Various Clinical Samples in a Tertiary Care Hospital

Carbapenems are one of the β-Lactam antibiotics with a broad spectrum of antibacterial activity. Inappropriate use of this antibiotic can produce resistance strains by production of carbapenemases enzyme. The Klebsiella pneumoniae carbapenemase (KPC) is the most important mechanism of enzymatic resistance seen in Enterobacteriaceae isolated especially Klebsiella pneumoniae. The aim and objectives of this study was isolation and detection of the Carbapenem resistant Klebsiella species strains with phenotypic methods. Out of 11248 samples, 602 Klebsiella species (33.7%), were isolated by standard Microbiological protocol & Antibiotic susceptibility test was performed by disk diffusion method with CLSI guidelines. A total 602 Klebsiella species strains were isolated. Of the 602 isolates of Klebsiella species, Klebsiella pneumoniae were 323 (54%) and Klebsiella oxytoca were 279 (46%). Antibiotic sensitivity pattern revealed maximum resistant to Cephalosporins (22%) followed by Ciproflaxacin (14%), Gentamycin (10%), Ceftriaxone Clavulanate (8%), PiperacillinTaxobactum (7%), Amikacin (7%).Out of 602 Klebsiella spp, Carbapenems resistant isolates were 28 (5%).All the 28 (100%) isolates were resistant to Meropenem and 23 (83%) isolates were resistant to Imipenem. Carbapenems are the drug of choice for multidrug resistant infections, like ESBL and AmpC producing isolates, but resistance to carbapenems by the production of carbapenamases and various other mechanisms has limited therapeutic options to use carbapenem drugs. K e y w o r d s